Journal: Bioactive Materials

Article Title: Bioprinting of live platelet-loaded nerve conduit using energy-dissipative hydrogel

doi: 10.1016/j.bioactmat.2025.11.021

Figure Lengend Snippet: Evaluation of nerve conduits in promoting early nerve regeneration. (A) Immunostainings of NF200 (green), S100-β (red), and DAPI (blue) in longitudinal sections of platelet-loaded PEGDA, GelMA, and F127DA conduits at 1 week post-surgery. (B) H&E staining images of regenerated nerve longitudinal sections at 3 weeks post-surgery. The immunofluorescence images of (C) NF-200 (red), S100-β (green), DAPI (blue), and (D) CD31 (red) at 12 weeks post-surgery. Percentage of (E) NF-200 and (F) S100-β positive areas in regenerated nerves (n = 3, technical replicates). (G) Blood vessel density in regenerated nerves (n = 3, technical replicates). Data are presented as means ± S.E.M. (standard error of the mean). ∗∗p < 0.01, ns means no significance.

Article Snippet: The nuclei were stained by DAPI for 5 min. Additionally, sections were incubated with rabbit anti-CD31 antibody (1:1000, Proteintech) using the same protocol to assess angiogenesis.

Techniques: Staining, Immunofluorescence

Journal: Bioactive Materials

Article Title: Bioprinting of live platelet-loaded nerve conduit using energy-dissipative hydrogel

doi: 10.1016/j.bioactmat.2025.11.021

Figure Lengend Snippet: Morphological evaluation of regenerated nerves after twelve weeks. (A) Immunofluorescence images of GAP43 (green) and DAPI (blue) in cross-sections of Sham, Auto, F127DA and PLT-F127DA groups. (B) Percentage of GAP43 positive areas in regenerated nerves (n = 3, technical replicates). (C) Immunofluorescence images of CD31 (red) and DAPI (blue) in cross-sections of all groups. (D) Blood vessel density in regenerated nerves (n = 3, technical replicates). (E) Photographs of myelinated nerves and (F) density of myelinated nerve fibers (n = 3, technical replicates). (G) TEM images of regenerated nerves. Quantitative analysis of (H) myelin sheath thickness (n = 3, technical replicates), (I) numbers of myelinated sheath layers (n = 3, technical replicates), and (J) axons diameter (n = 6, technical replicates). Data are presented as means ± S.E.M. (standard error of the mean). ∗p < 0.05, ∗∗p < 0.01, ns means no significance.

Article Snippet: The nuclei were stained by DAPI for 5 min. Additionally, sections were incubated with rabbit anti-CD31 antibody (1:1000, Proteintech) using the same protocol to assess angiogenesis.

Techniques: Immunofluorescence

Journal: Regenerative Therapy

Article Title: A temperature-sensitive chitosan hydrogels loaded with nano-zinc oxide and exosomes from human umbilical vein endothelial cells accelerates wound healing

doi: 10.1016/j.reth.2025.04.020

Figure Lengend Snippet: IHC study for the expression of CD31 was performed to assess angiogenesis in the wounds. (A) Representative images of immunohistochemical staining of CD31 in wound beds at 14 days after surgery. Scale bar, 50 μm. (B) Quantitative analysis of the number of new blood vessel per field (n = 6). ∗∗∗ P < 0.001.

Article Snippet: Immunohistochemistry with a rabbit anti-rat CD31 antibody (Wuhan Servicebio Technology, China) was performed on tissue sections to assess angiogenesis in the wounds following intervention [ ].

Techniques: Expressing, Immunohistochemical staining, Staining